Generation of a Stable Cell Line Producing High-Titer Self-Inactivating Lentiviral Vectors
نویسندگان
چکیده
منابع مشابه
Generation of a stable packaging cell line producing high-titer PPT-deleted integration-deficient lentiviral vectors
The risk of insertional mutagenesis inherent to all integrating exogenous expression cassettes was the impetus for the development of various integration-defective lentiviral vector (IDLV) systems. These systems were successfully employed in a plethora of preclinical applications, underscoring their clinical potential. However, current production of IDLVs by transient plasmid transfection is no...
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background: p19 cells are mouse embryonic carcinoma cells which contain pluripotent ability, like stem cells, to differentiate into different cell lines. there are several properties for this cell line that make it a valuable cell model for study of developmental stages. methods: at the first step, pts2-egfp coding sequence which was cloned in pucd2.hygro vector was used for transfection in to ...
متن کاملFactors influencing the titer and infectivity of lentiviral vectors.
Lentiviral vectors have undergone several generations of design improvement to enhance their biosafety and expression characteristics, and have been approved for use in human clinical studies. Most preclinical studies with these vectors have employed easily assayed marker genes for the purpose of determining vector titers and transduction efficiencies. Naturally, the adaptation of these vector ...
متن کاملMobilization and mechanism of transcription of integrated self-inactivating lentiviral vectors.
Permanent genetic modification of replicating primitive hematopoietic cells by an integrated vector has many potential therapeutic applications. Both oncoretroviral and lentiviral vectors have a predilection for integration into transcriptionally active genes, creating the potential for promoter activation or gene disruption. The use of self-inactivating (SIN) vectors in which a deletion of the...
متن کاملThe effect of age on hepatic gene transfer with self-inactivating lentiviral vectors in vivo.
It is known that cellular proliferation, by either compensatory regeneration or direct hyperplasia, can augment lentiviral vector transduction into hepatocytes in vivo. For this reason, the present study was designed to determine if adolescent mice (312 weeks of age), which still have relatively proliferating livers, would have differential transduction compared to older (7 weeks of age) mice. ...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2001
ISSN: 1525-0016
DOI: 10.1006/mthe.2000.0238